Application
Immunoblotting (1:1000; see comments)
General description
Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the A1-1 form (α-form) of glutathione S-transferase (GST).
This Anti-Glutathione S-Transferase A1-1 Rabbit pAb is validated for use in Immunoblotting for the detection of Glutathione S-Transferase A1-1.
Recognizes the α-form of GST. Does not react with µ- or π-GSTs.
Immunogen
recombinant human GST A1-1 (Cat. No. 354205)
Human
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Holmes, J., et al. 1990. Br. J. Cancer62, 209.
Specific for the α-form of GST. Does not react with µ- or π-forms of GST. For immunoblotting a dilution of 1:1000 will yield a positive result with ≥0.2 µg of human A1-1 GST, using alkaline phosphatase-conjugated anti-rabbit IgG as a secondary antibody. Variables associated with assay conditions will dictate the proper working dilution.
Packaging
100 µL in Plastic ampoule
Physical form
Serum diluted in PBS.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C).
Warning
Toxicity: Standard Handling (A)
This product has met the following criteria to qualify for the following awards: