Application

Immunoblotting (1:1000; see comments)

General description

Rabbit polyclonal antibody supplied as undiluted serum. Recognizes the A1-1 form (α-form) of glutathione S-transferase (GST).

This Anti-Glutathione S-Transferase A1-1 Rabbit pAb is validated for use in Immunoblotting for the detection of Glutathione S-Transferase A1-1.

Recognizes the α-form of GST. Does not react with µ- or π-GSTs.

Immunogen

recombinant human GST A1-1 (Cat. No. 354205)

Human

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Holmes, J., et al. 1990. Br. J. Cancer62, 209.

Specific for the α-form of GST. Does not react with µ- or π-forms of GST. For immunoblotting a dilution of 1:1000 will yield a positive result with ≥0.2 µg of human A1-1 GST, using alkaline phosphatase-conjugated anti-rabbit IgG as a secondary antibody. Variables associated with assay conditions will dictate the proper working dilution.

Packaging

100 µL in Plastic ampoule

Physical form

Serum diluted in PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Warning

Toxicity: Standard Handling (A)